- Terminology
- Pipetting techniques
- Low-volume dry plate pipetting.
- Volume calibration.
- Setting up liquid classes.
- How to reduce liquid retention in tips when using the same tips for multiple plates.
- Multi-pipetting (e.g. take up 15µl and dispense 5µl to all plates accurately).
- How to mix properly after pipetting
- Wash Cycles
- Dilution Effects with Buffers
- Reagent and chemical compatibility
- Overview of Validation and QC
- Color Dyes
- Decontamination
Liquid Handling Training Theory and Practice Workshop
Quick summary of topics
Goals of the course
- Familiarize you with the terminology and methods of automated liquid handling
- Discuss “optimal” pipetting techniques
- Learn about reagent and chemical compatibility issues
- Introduction to validation/QC testing options
- Review proper decontamination techniques
- Try hands-on operations on real liquid handling robots with an aqueous liquid class using a calibration kit modifying aspiration and dispense speeds with contact and non-contact dispensing handling “issues” (the majority of the class)!
Full Summary of Course Topics
Note: not all topics are listed and some topics might be subject to rotation.
- Introduction to liquid handling robots for HTS
- Fixed tips versus disposable tips
- Liquid handling terminology, issues and techniques
- Reagent and chemical compatibility and handling issues
- Dilution Effects in Assay Development
- Validation and QC techniques and options
- Decontamination protocols
- Hands-on pipetting on real robots modifying speed in delivering a Standard aqueous liquid class solution colored with scientific dye(s)
- Measuring CV precision
- Measuring CV accuracy
- Varying dispensing speed
- Varying Aspiration
- For contact and non-contact dispensing
- Disposable and fixed tips compare
Testimonials
“I loved the training at our facility. We were focused on “Real World” issues instead of a generic course. This really cut the time to validate our Curves.”
“It was great to use a variety of Handlers to see which one would be the best for us.”
“This was a very useful course for my new position”
